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Based on NN703, low molecular weight growth hormone secretagouges (GHSs) with a reduced number of hydrogen binding sites were designed by removal of the C-terminal amide group.
Simple and efficient sensors 1 and 2 possessing azo and nitrophenyl as signaling units and urea and thiourea moieties as binding sites were designed and synthesized.
For several genes, multiple putative promoters were identified by the program (up to 4 for ARRDC3) and primers surrounding the putative forkhead binding sites were designed.
The binding sites were designed to allow incomplete base pairing with miR-312, such that the 5'seeded' and 3' regions of the miRNA would be fully base paired, with a central unpaired bulge (Fig. 1D).
The oligonucleotides flanking transcription start site and predicted Tcf4 binding sites were designed within ~200-bp windows in the 700-bp upstream of the transcription start site (amplicon U), and in the 500-bp windows downstream of transcription start site (amplicon D) for detecting miR-181a-2 miR-181a-2 miR-181a-2er.
To confirm the presence of a putative binding site for miR-24-2 withen the 3'UTR region of the BCL-2 gene, the specific primers flanking the binding sites were designed and the resulting amplicon was cloned into the 3'UTR region of the luciferase gene of the reporter vector pGL3 (pGL3/ BCL-2).
Similar(54)
A simple turn-on fluorescent anion chemosensor with amide moiety as binding sites was designed, synthesized and characterized.
A 1,2,3-trioctyloxyphenyl-based organogel (G1) containing a pyrene fluorophore and urea-sulfonamide anion binding sites was designed and synthesized.
A novel fluorescent chemosensor HACBA with carbazole-hemicyanine fluorophore as signal reporter and N,N,N'-tri 2-pyridylmethyl)ethyleN,N,N'-tri 2-pyridylmethylg sites was desigN,N,N'-tri 2-pyridylmethyl
An organogelator G1 based on multi self-assembly driving forces, fluorescent and colorimetric signal groups and anions binding sites was designed and synthesized.
Putative FOXM1 DNA binding sites [67] within promoter regions (within 1 Kb upstream of gene) of CEP55 and HELLS were identified using ClustalW2 sequence alignment tool [68]) and qPCR primers flanking the putative binding site were designed (Supplemental Fig. S9).
More suggestions(19)
binding sites were assigned
binding sites were found
binding sites were sought
binding sites were included
binding sites were refined
binding sites were compared
binding sites were identified
binding features were designed
binding sites were isolated
binding sites were examined
binding sites were implicated
binding sites were blocked
binding sites were required
binding sites were studied
binding sites were used
binding sites were aggregated
binding sites were enriched
binding sites were mutated
binding domains were designed
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