As a potential application of the FI methodology, the possibility of sulfonamides detection through their competition with MB for the DNA binding sites was demonstrated.
High resolution detection of single RNA transcripts with 96 tandem repeats of the MS2 binding sites was demonstrated in E. coli by using dimeric MS2d fused to GFPmut3 (MS2d-GFP fusion protein) as a detection tag [ 18].
The differences between these two binding sites are demonstrated in Figure 2, which shows the structures of the insulin receptor tyrosine kinase (IRK) and the serine/threonine kinase Akt crystallized with ATP mimics bound to the ATP binding pocket and peptidic ligands bound to the substrate site.
Moreover, the zinc-dependent binding of purified Zur protein to double-stranded 40-mer oligonucleotides containing candidate Zur-binding sites was demonstrated in vitro by DNA band shift assays.
Already at the turn of the centuries, the presence of GnRH-binding sites was demonstrated in uterine myomas, suggesting direct effect of GnRH analogues upon the uterine tissues [ 19].
Highly efficient and specific displacements of both ligands from their endogenous mitochondrial binding sites were also demonstrated (data not shown).
Furthermore, direct interaction between delphinidin and ERα activator site is demonstrated using both binding assay and docking.
Notably, adhesion force maps demonstrated that binding sites were essentially located in the septum region, and more specifically on the equatorial rings (Fig. 6d), suggesting that newly formed peptidoglycan was inserted in these regions.
Randomly selected binding sites were tested for sequence and verified by ChIP coupled with real time PCR, demonstrating the existence of a GATA3 binding site and binding affinities corresponding to relative peak intensities (data not shown).
