Sentence examples for binding sites were constructed from inspiring English sources
Besides, mutant vectors containing four mutated bases on the predicted binding sites were constructed.
Unless otherwise indicated, all genetic tools (i.e., promoters, ribosome binding sites) were constructed as fusions to the EYFP reporter gene and cloned into pACSA_pcpcB_YFP.
COX-2 3´UTR luciferase reporters containing two putative miR-199a binding sites were constructed to validate the direct binding between COX-2 mRNA 3´UTR and miR-199a.
A non-enzymatic electrochemiluminescence (ECL) sensor based on the competitive reaction between glucose and phenoxy dextran (DexP) for concanavalin A (ConA) binding sites was constructed for detecting glucose.
The promoter region of Cricetulus griseus HMG synthase from −368 to +1 bp, containing SREBP binding sites, was constructed by Eurofins (Germany) with SmaI and XhoI restriction sites.
A position weight matrix (PWM) representing ribosome binding sites was constructed by running ELPH [ 81] on the 25 bp regions upstream of the predicted start sites.
To further confirm the direct regulation of FOXO1 by miR-1269, the pGL3- FOXO1-3'-UTR-luciferase reporter, containing the three putative miR-1269 binding sites, was constructed.
The benchmark is, indeed, the composition of three different tests, each of them related to the same binding sites, but differing in the way the sequences outside the binding sites are constructed.
Human CYP2E1 luciferase reporter containing putative p53 binding sites was constructed by amplifying the upstream region of the CYP2E1 promoter -7873 to -5896 (counted from the translation initiation site) and inserting it in the pGL3 promoter luciferase vector (Promega, Madison, WI, USA).
