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Its binding sites were confirmed by biotin-conjugated iodoacetamide assay and a SAR model was generated to guide further structural modification.
Predicted binding sites were confirmed by EMSA bandshift assays using an antibody specific for HNF4α.
Two point mutations along the miR-138 seed binding sites were confirmed by sequencing analyses.
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The importance of these WT1 and SP1 TFBS as candidate binding sites was confirmed by the in vivo ChIP assay.
The occurrence of both factors at common binding sites was confirmed by quantitative PCR using independent biological replicates.
Furthermore, multiple substrate-binding sites were confirmed in the single protein by high-resolution NMR spectroscopy, using partially purified TriCat enzyme.
The ability of this compound to interact with the AChE peripheral binding site was confirmed by kinetic studies and by molecular modeling investigation.
The intracellular location of this binding site was confirmed by FITC-NLX binding in intact A7 cells.
The sequences of predicted EGR2 sites were confirmed by comparison with potential EGR2-binding sites defined in (84) or (85).
In vivo, Stat92E binding of 3 n sites was confirmed for the even skipped (eve) gene (Small et al, 1996).
Functionally relevant binding sites were subsequently confirmed through chromatin immunoprecipitation.
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binding sites were blocked
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binding sites were calculated
binding sites were defined
binding sites were reported
binding sites were determined
binding sites were visualized
binding sites were shared
binding sites were selected
binding sites were overrepresented
binding sites were mapped
binding sites were analyzed
binding sites were generated
binding sites were associated
binding sites were used
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