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The two agonist binding sites were assumed to be equivalent and independent [37] and the interval durations at all three concentrations were fitted together by using a C↔AC↔A2C↔A2O kinetic model (A = agonist) that had four rate constants as free parameters: single-site association (k+, scaled by [A]), single-site dissociation (k−), ko, and kc.
Gelatinase binding sites were assumed to be within the rigid triple-helical collagen structure and thus far have been described only at the oligopeptide level [ 7, 16].
Similar(58)
Conversely, distant binding sites are assumed to have highly distinct binding properties.
SNPs with high conservation scores, high positive selection scores, or within microRNAs' target binding sites are assumed to have higher disease risk [8], [9], [10], [11].
The most commonly used method is a log-odds approach in which the frequencies of bases in the binding sites are assumed to be proportional to their contributions to binding affinity [9], [22].
In fitting all three data sets, a single binding site was assumed on VCA.
The dissociation of stable complex between transcription factor and binding site was assumed to be slow and was modelled by a stochastic transition with a relative rate of 10/a.u.u
The speed of a TF's binding to or dissociation from its target sites is assumed to be much more rapid than the transcription process, thus rapid-equilibrium approximation can be used.
If it could have been assumed that binding sites were in typical positions on the subsequence, then this result implies that the main thrust of our results could have been deduced without knowing the exact position of the binding site within the subsequence.
Numbers of AGO2 binding sites were counted if they are found in the vicinity of L1 assuming that L1 is located between any exons.
The total number of cortactin filament side binding sites was calculated by assuming a cortactin to F-actin subunit stoichiometry of 1 6.
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