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Based on these 12 predicted binding sites, we prepared 11 probes for EMSAs (see Fig. S10 and Table S4).
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To identify native TR-binding sites, we prepared chromatin from the TRα1 expressing tissues brain and heart of TRα1 GFP mice, and performed ChIP with an anti-GFP antibody.
To identify DNA sequence motifs of OmpR binding sites, we first used the MEME software tool28 with the genomic sequences of binding sites.
For RpoD or RpoS binding site identification, we prepared a ChIP library from cells harvested in exponential phase and stationary phase, respectively.
After prepared the 3D structures of proteins and ligands, and find out the binding site, we can perform docking for virtual screening.
The water soluble 43,000-dalton fragments of band 3 (the high-affinity ankyrin binding sites) were prepared from one of the patients and competed normally for 125I-ankyrin binding in solution.
ND analysed the TRANSFAC binding sites and prepared the data sets.
JB conducted alternative splicing experiments and bioinformatic analysis; MP conducted in situ hybridization experiments; RD identified transcription factor binding sites; AF prepared text and figures, and assisted with data analysis; AR supervised studies and assisted with data analysis.
We have modelled the FA and FB binding sites by preparing two apo-peptides (maquettes), sixteen amino acids each.
The Langmuir isothermal model and the Langmuir–Freundlich isothermal model suggest that ACV-MIMs have more homogeneous binding sites than MIPs prepared through normal imprinting.
ILs and β-CD was applied to provide multiple binding sites to prepare Lys SMIP and Fe3O4@DA was designed to make the product separate easily and prevent the aggregation of GO which could improve absorption capacity for its large specific surface area.
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