Sentence examples for binding sites we performed from inspiring English sources

To determine DNA-binding motif(s) that was enriched at the Atf1/Pcr1 binding sites, we performed the motif-discovery scan [44] using the 150bps sequence located at the apexes (see Materials and Methods).

Since the IGF-2 gene promoter contains multiple consensus STAT binding sites, we performed electromobility shift assays with nuclear extracts of untreated or PRL-treated T37i cells and the STAT response element located −3184 bp upstream of the transcription initiation start site of IGF-2 gene (exon 1) (Fig. 4F).

In order to gain insight into the mechanism of how cohesin is recruited to the ESC transcription factor binding sites, we performed a proteomic study on Nanog, the transcription factor with the highest enrichment score and the most similar expression pattern changes upon RAD21 depletion.

To investigate the sequencing depth necessary to saturate the number of detectable binding sites, we performed the following computational simulation.

To identify the critical binding sites, we performed a comprehensive binding assay that utilized mutants containing four random amino acid mutations spanning nearly all of the loop regions.

To gain more insight into the binding affinities of nuclear extracts to different probes representing the putative transcription factor binding sites we performed several EMSAs.

To identify groups of OR genes with highly similar binding sites, we perform a clustering analysis (see methods).

Besides merely identifying in vivo binding sites, we perform an extensive comparative analysis of in vitro, in vivo and in silico binding, exploiting the knowledge of the DNA-binding specificity model.

To determine whether γ-tubulin, similar to Dp1 (1), binds to the E2F DNA binding site, we performed an electrophoretic mobility shift assay (EMSA; ref. 31).

To test the functional consequence on enhancer blocking by the mutated binding site, we performed transfection experiments with episomes containing the F1F2 or the F1mutF2 binding sites.

In order to test if the K5 Hs II segment contains a bona fide p63 binding site, we performed Electrophoretic Mobility Shift Assays (EMSA).