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In order to predict putative AtoC binding sites, we implemented an ab initio motif detection procedure.
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For multiple adjacent binding sites, we generated the tags for each single binding site, and accumulated the tag counts at all positions from all binding sites.
To ensure the biological relevance of interaction partners and binding sites, we cluster similar binding sites of homologous protein complexes ('conserved binding site clusters').
To map the calpain cleavage site, we implemented two approaches.
To facilitate the detection of statistically significant differences in binding site dynamics, we also implemented a version where a subtree or subtrees can have different binding site turnover parameters (e.g., see fig. 1).
In order to compare signal transduction through a Grb2-binding site with signal transduction through a ShcA-binding site, we replaced the ShcA-binding site in the NGF receptor with a Grb2-binding site.
Multiple language bindings are supported for XPCOM; we implemented our extension in JavaScript.
To detect positive selection we implemented the site models.
However, it should be interesting to explore specific biological interactions with the model, such as receptor transphosphorylation, hetero/homodimeric receptors or allosteric competition between binding sites, which could be easily implemented and experimented.
The genomic sequences of the human homologs of the differentially expressed genes were selected, and regions between position 500 upstream and 100 downstream of the transcription start site were searched for putative transcription factor binding sites (TFBS) using F-match implemented in the Explain Analysis System (Biobase GmbH, Wolfenbüttel, Germany) [ 29, 30].
We implemented a model of receptor binding using a Monte-Carlo algorithm to simulate ligand diffusion and binding.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com