Sentence examples for binding sites we constructed from inspiring English sources

To be able to predict the location of the PBS (Primer Binding Sites), we constructed a database of tRNAs using tRNAscan-SE (version 1.3.1) (24).

To further isolate the dsDNA and ssDNA binding sites, we constructed fragments of the α subunit and performed stretching experiments in the presence of these protein fragments.

To estimate distances between binding sites, we construct a distance matrix for the 22 amino acid residues of each binding site, utilizing Grantham indices [26].

To test the specific regulation through the predicted binding site, we constructed a reporter vector consisting of the luciferase coding sequence followed by the 3′-UTR of Smad4.

In order to confirm whether the inhibitory effect of BMP-6 is dependent on the AP-1-binding sites, we constructed another set of miPPR-21s (Figure 2B).

In luciferase-reporter assays using a SHOX2 reporter-construct containing all putative binding sites, we could demonstrate that TBX5 acts cooperatively with NKX2.5 to activate SHOX2 expression.

We determined plausible binding sites between constructed CD4-bound gp120 trimer and homology modeled membranal CXCR4, and tested NeoR6 and Neo-r9 interfering with this interaction.

A non-enzymatic electrochemiluminescence (ECL) sensor based on the competitive reaction between glucose and phenoxy dextran (DexP) for concanavalin A (ConA) binding sites was constructed for detecting glucose.

Besides, mutant vectors containing four mutated bases on the predicted binding sites were constructed.

COX-2 3´UTR luciferase reporters containing two putative miR-199a binding sites were constructed to validate the direct binding between COX-2 mRNA 3´UTR and miR-199a.

The promoter region of Cricetulus griseus HMG synthase from −368 to +1 bp, containing SREBP binding sites, was constructed by Eurofins (Germany) with SmaI and XhoI restriction sites.