Sentence examples for binding sites was validated from inspiring English sources
Paupar occupancy at nine candidate binding sites was validated using CHART-qPCR in two further independent experiments (Supplementary Fig S4).
The protein binding activity at this site was validated but was not dependent on nitrate in the media [ 64].
The binding of SOX9 at this site was validated by EMSA and ChIP-qPCR.
As none of these binding sites was experimentally validated, some of them might be false positive.
These binding sites are experimentally validated and documented in the RegulonDB database [ 20].
Such binding sites were subsequently validated in detailed docking studies with known DAT ligands (56).
However, no corresponding miRNA binding sites was found in either CMBL or PIP4K2A from microRNA public database, future experiment will be performed to validate these results.
The putative binding sites were then further validated using JASPAR and ChampionChIP databases [22], and carefully selected for ChIP-qPCR analyses.
COX-2 3´UTR luciferase reporters containing two putative miR-199a binding sites were constructed to validate the direct binding between COX-2 mRNA 3´UTR and miR-199a.
Seven functional Sp1/Sp3 binding sites were detected computationally and validated experimentally within the first 500 bps upstream of the ATG of the MSH6 gene, and were shown to be required for its transcription.
