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A similar result was observed when 450-stop, which did not contain any predicted tensin binding sites, was used (Fig. 2B).
pFOPflash containing mutated TCF binding sites was used as negative reporter control.
For the studies presented here, a new pGL4 reporter with decreased cryptic DNA regulatory elements and transcription factor binding sites was used as a backbone for reporter.
MG-Luc plasmid (kindly provided by Dr. Bert Vogelstein) carrying a mutation in the p53 binding sites was used as a negative control [ 22].
(1255) HSA, immobilized in a way that maintains accessibility of the binding sites, was used to determine the binding of chemicals by liquid chromatography, capillary electrophoresis, 1268) and surface plasmon resonance analysis.
A total of 223,000 binding sites was used to analyze whether the presence of a known TFBS at a given position in the promoter determines the acetylation level at that position.
Similar(51)
Experimental and predicted binding sites are used in molecular docking with the default and optimized box sizes.
The 150bps sequences at the Atf1/Pcr1 binding sites were used in DNA-binding motif discovery.
Multiple copies of the miR-312 binding sites were used to increase the probability of efficient miR-312 binding.
As for ligand-based methods, structural descriptors of ligand-characterized binding sites are used to transfer the ligand annotation of putative targets to the query binding site.
Validated CoREST and REST promoter binding sites were used as positive controls, while a non-target gene promoter was used as a negative control.
More suggestions(19)
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