PACAP binding sites were studied using a radioreceptor assay with 125I-labelled PACAP in various tissues.
Rat brain guanosine binding sites were studied by (i) a pharmacological approach to confirm the hypothesis of the existence of specific G-coupled receptors for guanosine (1) and, for the first time, delineate a structure activity relationship for a series of guanosine derivatives; (ii) a molecular modelling approach to design a pseudo-receptor construction.
Additionally, the properties of binding sites were studied for mRNA sequences of both human and other animal species.
The initial rate response of SULT1A1 to nucleotide binding at the high- and low-affinity sites was studied using 1-HP, a fluorescent acceptor.
Comparisons over the binding sites were specially studied to improve the discussion about the selectivity of potential new drugs.
However, polymorphisms in coding regions resulting in a non-conservative amino-acid substitution in conserved regions of the genome, or in potential transcription factor binding sites, are also studied because of their potential functionality.
The yeast study with TF binding sites was not confounded by some of the factors mentioned above, since most of the regulatory sequences were experimentally determined, and a comprehensive set of DNA binding sequences for almost all of the yeast TFs were available [ 67].
The two undecanucleotides (11-mers) corresponding to the XerC and XerD binding sites were designated in this study as dif XerC and dif XerD, respectively, whereas the central hexanucleotide between the two Xer binding sites was named as dif cent (Figure 1A).
The residues identified as binding sites have been studied in terms of binding segments.
A blast search for each primer pair verified that the binding sites were unique for each gene studied.
