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Prediction of kinase binding sites was performed with http://scansite.mit.edu/motifscan_seq.phtml.edu/motifscan_seq.phtml
Prediction of the putative transcriptional factor binding sites was performed using Transfac software.
Analysis of transcription factor binding sites was performed using GENOMATIX software (http://www.genomatix.de).de
Confirmation of the predicted binding sites was performed using Quantitative real-time PCR (Q-PCR).
Search for conserved transcription factor binding sites was performed by combining the PromAn, TFSearch and MatInspector programs [39], [40].
An analysis of individual TFBS with a test for pair wise co-occurrences of binding sites was performed.
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The 5′ upstream region of Os04g27430 in RH, KD, and IL162 was sequenced (accession nos. KC511031, KC527594, KC511035), and searches for transcription factor (TF) binding sites were performed (http://www.cbrc.jp/research/db/TFSEARCH.html).
Detection of binding sites is performed mapping back the sequenced fragments to the genome (Fields, 2007).
Promoter analysis for putative CEBP binding sites were performed using Genomatix MatInspector software, release 7.7(3) (Genomatix Software GmbH, Munich, Germany).
The whole genome screening of all possible CRP binding sites were performed using an algorithm that we have developed previously [ 32].
For the question of the evolution of gene regulatory elements alignments of the binding sites were performed and introduced into the system.
More suggestions(15)
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