Sentence examples for binding sites was mutated from inspiring English sources
Moreover, the inhibition was partially rescued when one of the binding sites was mutated or almost fully relieved with all sites mutated.
These sites were confirmed in vitro: the repression of MET upon ectopic expression of miR-34c in PC3 cells (P=0.044) was abolished if either of the two predicted binding sites was mutated, as measured using luciferase reporter assays with the 3′-UTR of MET, see Figure 2C, indicating that both sites are needed for the repression of MET to occur.
When three or all four miR-137 binding sites were mutated simultaneously, the mutated vector alone was unable to express the luciferase gene (data not shown).
Full-length angiocidin bound polyubiquitin while three angiocidin recombinant proteins whose putative polyubiquitin binding sites were mutated either failed to bind polyubiquitin or had significantly diminished binding activity.
This repression does not decrease if the distal of the two binding sites is mutated (as the proximal site can still block transcription), but mutation of the proximal site results in a significant de-repression and loss of both sites completely abolishes transcriptional control by CysR.
Three 5′-flanking regions of the rat Vegf gene; 1) the construct including both C/EBPβ binding site and HIF1α binding sites (−1171 bp to +115 bp), 2) the construct lacking the C/EBPβ binding site (−976 bp to +115 bp) and 3) the construct that four HIF1α binding sites are mutated (−1171 bp to +115 bp), were amplified using the primer sets in Table 1.
