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A 50 bp module encompassing the PBX1, PAX2, and MEIS1 binding sites was excised from the wild type CNE5 fragment.
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The artery and all side branches between two ligation sites were excised completely.
Upon Cre recombination, the flanking lox sites are excised and the GFP region is flipped, resulting in GFP expression.
If a heat shock is performed in this triple transgenic line, the yellow gene flanked by FRT sites is excised.
PCR products containing integration sites were excised from the gel and extracted using the QIAGEN Gel extraction kit (Qiagen, Hilden, Germany).
ORFs containing modified restriction sites were excised from pJET cloning vector and ligated into pre-digested, purified pGEX4T-2.
Then the generated AP site was excised by endonuclease IV (endo IV), making the PA probe form a RCA amplicon through reconformation.
These binding sites are highly charged.
Noteworthy, these binding sites are juxtaposed to GATA3-binding sites.
The HindIII- EcoRI fragment containing the minimal hsp70 promoter with five GAL4 binding sites upstream of it was excised from the pUAST vector (26) and cloned into the pBluescript SK + vector between lox sites to produce the lox UAS) plasmid.
One possibility is heightened awareness or surveillance of the body site where the first tumour was excised.
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CEO of Professional Science Editing for Scientists @ prosciediting.com