Sentence examples for binding sites was done from inspiring English sources

To visualize the HA tag, whole oocytes expressing KCNQ2-HA were fixated in 4% formaldehyde (37%) in Ca-free ND96 solution for 15 min, 3 days after the injection of mRNA.Blocking of nonspecific binding sites was done by 5% skim milk for 1 h.

Search for potential transcription factor binding sites was done using the MatInspector program [ 36].

Detection and annotation of binding sites was done as described earlier (7).

In silico prediction of putative GLI binding sites was done using the ScanAce algorithm (Roth et al, 1998).

The verification of the predicted binding sites was done using experimentally identified regulatory regions from the Encode project [ 9].

Site-directed mutagenesis of GLI binding sites was done using the QuickChange XL site-directed mutagenesis kit (Stratagene) according to manufacturer's instructions.

The first study to elucidate the CD36-FA binding site was done by Baillie et al, 13 suggesting a potential site by utilizing a simple sequence alignment and without any validation.

Identification of conserved STAT1-binding site was done using the rVista 2.0 web utility.

To further determine the possibility of NF-κB binding to MMP-2/MMP-9 promoter binding sites, EMSA was done with specific oligonucleotides As shown in Figure  6C, the TGF-β1-stimulated NF-κB DNA binding activities were strongly inhibited by pinocembrin at the concentration of 5 μM.

Prediction of ribosome binding sites (RBS) was done using RBSfinder [31] algorithm developed by TIGR (http://www.tigr.org/software/genefinding.shtml).shtml

Accordingly, the identities of genes that contain C/EBPδ binding sites are diverse and don't immediately describe shared functions (Supplementary Data Set 1).