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Overlap with p63 binding sites was determined using the "bedtools intersect" command.
The genomic region to be examined for transcription factor binding sites was determined using BLAST2 [ 50] and FirstEF [ 51].
Conservation of the binding sites was determined using web-based CEAS software of the Cistrome/Galaxy platform [ 37].
The genomic location of detected binding sites was determined and considered as putative Zur box within −500 nucleotides relative to predicted translation start sites (TLS).
The number of binding sites was determined to be 951 ± 43 fmol/mg protein (n = 3), and the equilibrium dissociation constant of I-CYP for these binding sites was 32.4 ± 1.1 pM (n = 3).
The number of DIDS (4,4′-di-isothiocyanato-stilbene-2,2′-disulfonic 4,4′-di-isothiocyanato-stilbene-2,2′-disulfonic 4,4′-di-isothiocyanato-stilbene-2,2′-disulfonic 4,4′-di-isothiocyanato-stilbene-2,2′-disulfonic 4,4′-di-isothiocyanato-stilbene-2,2′-disulfonic 4,4′-di-isothiocyanato-stilbene-2,2′-disulfonic
Similar(54)
The binding strength and the number of binding sites were determined at different ionic strengths.
Furthermore, the inhibitory, divalent, metal ion binding sites were determined by NMR techniques.
Putative Smad binding sites were determined in the 4 kb upstream sequence from the transcription start site of mouse Olig2 using MatInspector and rVista2 [37], [37].
Locations of miRNA binding sites were determined using the mRNA sequence of mouse Mitf 3'UTR and are numbered starting at the first nucleotide after the stop codon.
The available surface ligand binding sites were determined with [H]granisetron.
More suggestions(18)
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