Sentence examples for binding sites was amplified from inspiring English sources

A 4-kb fragment of the 4.8-kb human TFGBR1 3'UTR (containing both putative let-7c binding sites) was amplified by PCR applied on genomic DNA extracted from HEK293 cells, using primers: 5'- CCAAGTTTAAACAGATCTGCTCCTGGGTTTTA (PmeI), and 5'- CCAAGCTAGC-ACCGGTATGCTCTGACAAATATTAAAC (NheI, AgeI), and cloned into the PmeI/NheI sites of pmirGLO, to generate pmirGLO-TGFBR1-long 3'UTR.

The human TRAIL promoter region containing the Sp1 binding sites was precipitated using either the anti-Sp1 antibody or IgG, and using the probe 1 (forward) and the probe 5 (reverse) shown in Figure 5A as gene-specific primers and the sequence (136 bp) encompassing the Sp1 binding sites was amplified.

The fragment containing three RUNX3 putative binding sites was amplified using the forward primer, 5′-GGGCAGAGCAGAGCTACAAATG-3′, and the reverse primer, 5′-AACCCAGCTCTAGCCAGCAGGT-3′.

The sequence located between the F3 and B3 primer binding sites was amplified using primers OP1 (5′-CACCGCCAGCGTTCCCTAT-3′) and OP2 (5′-AACTGCTGAAATGGCTGGAC-3′).

A region of the human FOXO1 3'-UTR containing three miR-1269 binding sites was amplified by PCR and cloned into vector pGL3 (Promega, Madison, WI, USA).

Of the several putative TRα and TRβ binding sites identified, a region containing both putative TRα and TRβ binding sites was amplified, and a second region from −184 to the transcriptional start site was amplified.

PPARγ/RXR ChIP-PET binding sites were amplified from 3T3-L1 genomic DNA by PCR and cloned into the pGL4-TATA vector (a minimal TATA box upstream of pGL4- Basic) by homologous recombination using the In-Fusion CF Dry-Down PCR Cloning kit (Clontech, http://www.clontech.com).

However, due to the simultaneous binding of the primer on both DNA strands, the sequence between the two binding sites is amplified.

rpoS were constructed as follows: The rhlR and rpoS ORFs including native ribosomal binding sites were amplified from PAO1 genomic DNA by PCR.

Fragments of the 3′UTR of RTEF-1 containing putative or mutated miR-125a-5p miR-125a-5p miR-125a-5pified bindingCR usitesspecific primers (Table S2).

The human miR-181c/d promoter sequences containing the Egr-1 binding sites were amplified by PCR using the following primers: (forward) 5′-ACAGGTCAAAAGCGACGGGG-3′ and (reverse) 5′-TCACGGTGGTGGCAACGGAA-3′.