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Interestingly, binding sites unique to either PU.1 or Spi-B demonstrated distinct profiles.
Specifically, binding sites unique to K562 cells show reduced nucleosome occupancy relative to the same genomic loci in GM12878 cells.
Therefore, we determined the epigenetic profiles of the promoters bound by Kaiso in both GM12878 and K562 (mostly promoter proximal) and of the sets of Kaiso binding sites unique to K562 cells that were promoter proximal versus promoter distal.
There is huge variation between individual ESR1 ChIP experiments with most binding sites unique to a single experiment.
On average, GR binding sites unique to A549 do not show nucleosome depletion in RS4; 11 (Fig. 1b, top panel).
The binding sites unique to miRNAs are located in the 5′-untranslated regions (5′UTRs), the coding domain sequences (CDSs) and the 3′-untranslated regions (3′UTRs) of mRNAs.
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Even when restricting analysis to only those studies conducted in MCF-7 cells exposed to estradiol for 45 minutes, 69.1% of binding sites were unique to a single dataset.
Of these 11 families, only CBNAC, TGA2 and WRKY11 binding sites are unique to one hour roots.
Predicted transcription factor binding sites included both unique and common motifs (Table 1).
We determined that the NFAT binding site is unique to humans; it arose by point mutation along the lineage separating humans from other great apes.
If a true binding site is unique to a genome, then it will not score high.
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