Sentence examples for binding sites to test from inspiring English sources
Nevertheless, we identified too many potential binding sites to test experimentally.
Real-time quantitative PCR was performed with previously known binding sites to test the enrichment of the immunoprecipitated (IP) DNA library [ 66]. qPCR and amplification of DNA was carried out according to the method of Cho et al. [ 66].
Here, we queried the UniProt database to determine the functions of TFs [ 26], and investigated the nucleosome occupancy around activator and repressor binding sites to test whether the functions of bound TFs influenced the nucleosome occupancy distribution in human cells.
We further tested all 10 up-regulated miRNAs for free-energy of 70 nt of 5´ and 3´ of the predicted binding sites to test whether the predicted binding sites are located in a region of very high free energy, suggesting a locally accessible site.
PAGE-purified primers containing the binding sites to be tested were ordered to produce 5′- and 3′-sticky ends for ligation into pMoPac16 (Supporting Information).
Our data regarding the extent of UTR coverage, as well as the significant chance of transcript overlap, provides insights that may help guide selection of sites more likely to be trans-acting factor binding sites to be tested in these studies.
I therefore propose to use the distribution of changes in strength of transcription factor binding sites to develop tests for natural selection on regulatory regions where the binding sites have been identified.
This reports highlights a mechanism that immune cells such as cytotoxic T cells might utilize to upregulate TRAIL and underscores the need to directly test binding sites to accurately delineate transcriptional regulation.
Here I consider the 5 Bcd binding sites and 6 Kr binding sites to illustrate the application of the test for selection to a complex regulatory region (see Additional file 1 for alignments of these binding sites).
None of these constraints are present for tests that compare binding sites to surrounding regions or synonymous sites [ 16, 34] or for tests of natural selection based on spacing between conserved blocks [ 35- 37].
We then made use of a − 350 nucleotides RCAN1-4 promoter construct containing a point mutation in one of the NFAT binding sites (− 350ΔNFAT) to test whether NFATc binding to this promoter is involved in the activation of RCAN1-4.
