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1H NMR identified the binding sites to be the imidazole nitrogen, the two-amide nitrogens and the terminal amino group.
In contrast to in vivo display systems, the in vitro approach allows all the capsid binding sites to be occupied by the 130-kDa PA-Hoc fusion protein.
Furthermore, sequence alignment of human, mouse, and rhesus BST-2 promoters showed STAT binding sites to be conserved, indicating a likely dependency on STAT3 for effective BST-2 gene expression22.
In 2015, Dr. Mann and his team discovered that the Hox transcription factors were also binding at many other locations — just more discretely at so-called 'low-affinity sites.' The scientists believed these low-affinity binding sites to be key to the Hox transcription factors being able to drive one aspect of development versus another.
This windowing allows closely linked binding sites to be considered simultaneously.
However, to increase sensitivity and the amount of polymorphism available for analysis, we did not require the binding sites to be aligned but just that they appear anywhere in each orthologous 3' UTR.
Similar(29)
Furthermore, many classes of RNA binding proteins require the binding site to be single-stranded.
These results identified the binding site to be in the C-terminal half of the molecule.
We usually allow a binding site to be shifted by two bases to its left and right, respectively.
We built two complementary models of the SigB binding site to be used in parallel for all subsequent analyses.
This requires the R15 binding site to be saturable, but we have no evidence for the latter.
More suggestions(15)
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