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However, the LTR contains two other Sp binding sites that bind the Sp factors shown to recruit HATs to the LTR.
This point was studied in detail in the case of the decidual prolactin gene, dPrl, where modifications after insertion provided multiple transcription factor binding sites that bind factors that interact with each other [ 206].
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One of these enzymes (ME1) (56501) is characterized by a dinucleotide binding site that binds NAD rather than NADP.
Enrichment of 5-hmC gains was greater at or near unique HIF-1 binding sites (sites that bind HIF-1, but not HIF-2) than at unique HIF-2 binding sites (sites that bind HIF-2, but not HIF-1) (Schödel et al., 2011).
Thus, T-KDE is capable of identifying constitutive binding sites that are bound by a protein either directly or indirectly.
Furthermore, although computational methods can successful identify binding sites that are bound by a particular TF in vitro, most of the predicted binding sites are not functional in vivo (Li et al. 2011; Whittle et al. 2009).
Our subsequent identification of STAT1 binding sites that are bound by RNApolII near the start positions of the IRF1 and IFNaR1 genes in non-stimulated cells suggested that basal levels of mRNA transcription might be detectable at these sites.
Recently, we carried out a comprehensive analysis of the 116 CTCF ChIP-seq datasets from 56 cell lines from the ENCODE (Encyclopedia of DNA Elements) consortium and identified ~24,000 CTCF binding sites that were bound in more than 90% of the 56 cell lines [ 3].
The GFP-tagged mRNA contains eight MS2 binding sites that can each bind a maximum of two (45 kDa) GFP-fusion proteins.
Applying fpocket cavity detection to the sc-PDB dataset (2013) to assess the impact of incorporating objective and unbiased cavity detection to BSC compared with only defining binding sites that surround exemplified bound ligands.
These authors also noted a large number of HSF binding sites that were also bound by BEAF prior to HS with a higher occurrence of the overlap taking place at promoters than within genes.
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