In silico prediction of putative transcription factor binding sites supports the hypothesis that CGB1 and CGB2 gene products are expressed in, and may contribute to, implantation and placental development.
Strikingly, 13 genes contained VDREs but no TCF/Lef consensus binding sites, supporting the concept that nuclear β-catenin can activate vitamin D/VDR target genes in the absence of TCF/Lef.
The identification of two variable lead scaffolds with differential binding sites supports the possibility of developing specific inhibitors of P. aeruginosa phospholipase D, PldA.
Fifth, 39 of the 42 MFA peaks center on ORC1/CDC6 binding sites, supporting the proposed function of ORC1/CDC6 as an initiator protein.
While the matrix similarity allows the assessment of the structural quality of a binding site, the number of conserved binding sites supports the possibility that these binding sites might be functionally relevant (62).
Independent qPCR experiments confirmed differential expression of 5 of the 6 TregHAR signature genes with predicted BACH2 binding sites, supporting the notion that harmine enhances Treg cell differentiation at least in part by modulating BACH2 signaling.
Similar to the binding described above, substitutions to the complementary face of the 5-HT3B suBunit (B–) produce receptors with recovery rates more similar to those from 5-HT3A recontainingntaining only A+A− binding sites, supporting the hypothesis that the interaction of VUF10166 at an A+B− interface is responsible for the observed differences between the homomeric and heteromeric receptors.
The log2MRIPA ratios (transformed representation of average protein abundance measured by iTRAQ) of the individual α4 and β2 nAChR subunit proteins were significantly correlated with the measured [H]-epibatidine binding sites, supporting the accuracy and reproducibility of iTRAQ-coupled LC MS/MS.
The recently determined structure of the β2AR in an activated state revealed surprisingly subtle changes in the orthosteric binding site, supporting the idea that agonist binding and activation requires only modest conformational change in that region.
Analysis of ChIP-seq transcription factor binding sites shows clustering of ZNF217 with FOXA1, GATA3 and ERalpha binding sites, supported by the enrichment of corresponding motifs for the ERalpha-associated cis-regulatory sequences.
