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Adenine, with its two metal binding sites, should show the best affinity, whereas thymine, with no strong metal binding sites, should show little or no affinity.
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Since ChIP-DNA fragments are equally likely to be sequenced from both ends, the tag density around a true binding site should show a bimodal enrichment pattern, with forward strand tags enriched upstream of binding sites and reverse strand tags enriched downstream of binding sites [ 10, 11, 35, 37].
Sterical demand close to the phosphate binding sites should inhibit coordination to the nucleobases.
Intuitively, constitutive binding sites should be biologically functional.
These binding sites should not be dismissed, but rather should be the focus of additional studies.
At a fixed concentration of Fluoro-probe, inhibithat that bind to the FPP binding site should compete with Fluoro-probe for binding to UPPS and show a competition profile similar to that illustrated in Figure 3A.
Its binding site should be well characterized.
This would allow SEA-DR1 complexes to bind transiently to the DR1 on the chip via the second binding site on SEA, whereas SEH-DR1 should show little or no such binding.
The 3D patterns corresponding to the quercetin binding sites are shown in green.
Their corresponding binding sites are shown in Figure 1.
The DScore binding affinities of curcumin and the corresponding binding sites are shown in Table 1.
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