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The chromosomal locations of AGO2 binding sites were retrieved from the CLIPZ database [68], which releases RNA-binding protein (RBP) binding site data generated by cross-linking and immunoprecipitation (CLIP) mapping technique.

Sites within 100 bp of predicted NR2E1 binding sites were retrieved from the oPOSSUM database.

Predicted transcription factor binding sites were retrieved from MSigDB (Subramanian et al., 2005; Matys et al., 2006).

Experimentally characterized human transcription factor binding sites (TFBS) were retrieved from the Professional release 11.3 (9/10/07) of the TRANSFAC database [ 18].

In addition, consensus sequences of TF binding sites (TFBS) were retrieved from two publicly accessible comprehensive plant cis-element databases, PLACE [ 11] and AtcisDB [ 12].

Binding site similarity methods: these methods compare entire binding sites and retrieve sets of proteins which share globally similar binding patterns.

Promoter binding was tested by PCRpolymerase chain reaction using primers spanning the upstream regions of miRNA203 and miRNA34a start sites, sequences retrieved from UCSC genome browser (primer sequences available upon request).

First, 41,013 TF-gene binding pairs were retrieved from the YEASTRACT database [ 14].

The HMMER signature files (profiles) of known DNA binding domains were retrieved from Pfam [ 68].

Finally, the number of PCR amplicons with unique shear sites was retrieved from deep-sequencing data.

The genome coordinates for conserved sites were retrieved from the table named phastConsElements17way.

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