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The effect of the initial of dye concentration factor depends on the immediate relation between the concentration of the dye and the available binding sites on an adsorbent surface (Salleh et al. 2011).
The effect of the initial dye concentration depends on the immediate relation between the dye concentration and the available binding sites on an adsorbent surface (Gautam et al. 2014b).
In the double trapping nanometry (dumbbell assay), preferred binding sites on an actin filament appear every 36-nm along the helix [35].
Here, we treat TFs and their binding sites on an equal footing and so, for example, the substitution rate in each is the same.
These results indicated that both heads have a propensity of searching for the energetically favorable binding sites on an actin filament, but the leading head does that more often because its binding affinity is diminished due to the molecular stress (enabling the next forward step) that it features.
While an allosteric response was originally believed to result from a large structural change elicited by binding of a small molecule to a symmetric oligomeric protein, communication between two distinct binding sites on an allosteric molecule can be mediated by small structural rearrangements or a change in its dynamic properties (12, 47 51).
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Taking advantage of the site-directed nature of Tethering, one can investigate the 'druggability' of particular binding sites on a protein surface; furthermore, Tethering has been used to find new binding sites and to stabilize allosteric conformations.
By taking into account the spatial arrangement of binding sites on a target protein, high-affinity multivalent ligands that geometrically complement their target can be designed and synthesized.
Our findings show that, once bound to the ATP synthase, probably through different binding sites on a common binding region on FO, the macrolide antibiotics oligomycin, venturicidin and bafilomycin behave as enzyme inhibitors.
In a paper to be presented at this conference (Apaydin et al., 2002b) we have also applied SRS to ligand docking in which we calculate the dynamics of ligand-protein association and dissociation in the region of various binding sites on a number of proteins.
The proposed ELISA involved a competitive binding reaction between ROS, in plasma sample, and the immobilized ROS-BSA for the binding sites on a limited amount of the anti-ROS antibody.
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