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This work demonstrates that using the competition of target protein with an ECL signal compound Ru bpy 32+ for binding sites of special aptamer confined on the electrode is promising approach for the design of label-free ECL aptasensors for the determination of proteins.
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Obviously, the described above stepwise binding mechanism, where intrinsic disorder of some proteins allows them to interact with hidden binding sites of ordered partners, represents a special case of a more general allosteric mechanism, where the complex formation between an IDP and its target leads to conformational changes in a target and opening of a hidden binding site.
Here in special, motifs which are involved in the consensus pattern of retinal binding sites of Pfam receptor families.
Predicted ligand binding sites of YD1.
The putative binding sites of KmMig1p are shaded.
(B ) The Ca2+ binding sites of LapG.
These residuals are not only the binding site of glucose but the binding site of the substrate, so changes of the special residuals cause difference in degree of bond to glucose.
(D) Comparison between adamantane binding site and HMA binding site of HCV p7 channels.
From the current response of ARS after site competition, binding site I of BSA was identified as the binding site of BSA to ARS.
≡SZn denotes the surface binding site of ZnS.
An example binding site patch is shown in Fig. 1 for the cofactor binding site of CDK2 [14].
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