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Unlike the binding sites of mAb LOM-8.7, the binding sites of mAb URA-1 did not overlap with CD11b or F4/80 in the medullary sinus.
Distribution of binding sites of mAb URA-1 was restricted to the cortical areas (Figure 2A).
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Usually, an intrinsic affinity in the (sub nanomolar range is observed for nanobodies and the antigen binding site of mAbs to their cognate antigen.
By screening 24 6-HB variants with single mutations at the b, c, and f positions in the helical wheels, we found that the critical binding sites of these mAbs were localized in the N-terminal region of the NHR and the C-terminal region of the CHR.
Other applications utilize uaas as photoactivatable cross-linkers to map the binding sites of peptides, mAbs, or small molecules with GPCRs.
Distribution of binding sites for mAb LOM-8.7 was mainly observed in the medullary and subcapsular sinuses, which was a similar distribution seen by MGL1/2-cross reactive mAb LOM-14 previously (Figure 2A).
Therefore, it was of interest to determine whether or not shedding of gp120 from virus particles was required for the CAP induced exposure of binding sites for mAb NC-1 on gp41.
In combination with transmission electron microscopy, Mab also permit direct visualization (via Mab binding sites) of specific carcinogen-modified structures in individual DNA molecules.
To further characterize the binding sites of V3-specific neutralizing monoclonal antibodies (mAbs) generated by immunizing mice with SU-Fc [36], [42], [43], ELISA assays were performed with the same set of SU-Fc proteins used in the binding studies (Table 4, Table 5).
Predicted ligand binding sites of YD1.
Most mimotopes obtained from phage displayed peptide libraries can be employed to facilitate the identification of novel peptide sequences that mimic binding sites for mAbs (Mayrose et al., 2007).
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