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Only the miRNA binding sites (indicated by blue horizontal lines) surrounded by 20-nt sequences at both ends were shown.
Interestingly, the majority of these genes contain FoxH1/Smad2 binding sites (indicated by an asterisk in Figure 5) adding supporting evidence that they are direct Nodal targets.
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In silico prediction of putative transcription factor binding sites indicated that of the four transcription factor binding sites potentially affected by the deletion, some are predicted to involve proteins not expressed in the breast.
Motif analysis was performed by using CisFinder tool (Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, NIH, Baltimore, MD, USA) [ 43] on 200-bp sequences centered at the expected binding site indicated by peak summit calculated using the MACS tool.
Mutant derivatives RLuc-MD1-Δ133 RLuc-MD1-Δ133 RLuc-MD1-Δ133tanded RLuc-MD1-Δ135 RLuc-MD1-Δ135 RLuc-MD1-Δ135g sites indicated in Figure S1 by inverse PCR.
Assays for the transcription regulatory function of NKX3.1 binding sites indicated repressor function that was disrupted by specific mutations affecting the DNA recognition of NKX3.1 transcription factor.
Both PNPLA6 and PNPLA7 contain two putative cyclic nucleotide binding sites indicating that they may be regulated by either cAMP or cGMP.
Of the 186 major Sty1 recruitment sites, 152 (∼82%) were found to overlap with the Atf1/Pcr1 binding sites (89 overlapped with the major Atf1/Pcr1 common binding sites and 63 with Atf1 and/or Pcr1 binding sites), indicating that Sty1 is primarily recruited at the Atf1/Pcr1 binding sites in the genome induced by H2O2 treatment.
Ultimately, we tested the Sp1 binding site indicated at position -99 by in silico sequence analysis.
(D ) Close-up of the MELTP binding site indicating the role of ScBub1R314 in MELTP binding.
Their potential binding sites, as indicated by the iCLIP data, are shown in Fig. 4C.
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