Sentence examples for binding sites in two from inspiring English sources

The fact that yeast display based selections enabled the construction of pH-dependent binding sites in two different scaffolds highlights the potency of this technology for this task in protein engineering.

Having demonstrated the functional significance of Slou-preferred binding sites in two FC enhancers, we next asked whether FCI-HD-preferred binding sequences are more generally involved in the regulation of FC gene expression.

Based on the resultant frequency of the matrices of the TFBS, the similarity of genes was determined by using Jaccard's algorithm, which does not consider the absence of binding sites in two promoters as an indication of similarity [ 38].

These results are in agreement with previous findings of a closed and less solvent-accessible active site in response to nucleotide binding.[ 14a] Overall, we detected three potassium binding sites in two proteins by using NH4Cl and N-edited NMR spectra.

A few other studies have made the same efforts to develop programs, DBChIP [ 12], DIME [ 13], to detect differential binding sites in two samples, but these programs suffer from the same drawbacks either lacking distinguishing binding patterns or neglecting the normalization between samples.

Allowing p to be the proportion of gain/loss of the miRNA binding sites between the duplicated genes, we have p = 17/(12 × 2 + 17) = 0.415, where 17 is the gain or loss number of the miRNA binding sites, 12 × 2 is the number of conserved miRNA binding sites in two paralogs.

To directly test this, we used CRISPR/Cas9 genome editing to delete core CTCF binding sites in three loci, including the CTCF site in the Sox2 super-enhancer.

The presence of at least two functional binding sites in one single trimerbody molecule was further demonstrated in bispecific trimerbodies.

The strategy used here could also be applied to the independent modification of binding sites in single chain avidin (scAvd), which has four binding sites in one polypeptide chain [18].

In addition to E2F family members that warrant investigation (above), we have identified c/EBPβ, FOXC1, FOXJ2, GATA-3, POU2F1/AP-2γ, RARA, TFAP2C, and TFE3 as estrogen-responsive transcription factors with predicted binding sites in one or both promoter regions of the PCNA gene.

We explored the reasons for this by mutagenizing putative binding sites in three cis-regulatory modules.