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Twelve AR binding sites identified from ChIP-Seq were tested for enrichment by real-time quantitative PCR.
Additionally, the MACS binding (p-value) score was concordant (R = 0.87, P = 0.00025) with the enrichment values from qPCR. 12 AR binding sites identified from ChIP-Seq were tested for enrichment by real-time quantitative PCR.
Importantly, AR binding sites identified from R1881 sample were no less conserved than those from R1881 sample, revealing that even with low levels of androgen, AR binding is far from random and likely occupies functional sites.
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There are four Ca2+-binding sites identified from CRDs of different species, among which the Ca2+-binding site 2 is structurally conserved and involved in carbohydrate-binding activity [15].
Unexpectedly, the major binding site identified for Nob1 was located in H40, distinct from the cleavage site.
Gradually eliminating low-affinity binding sites identified by NRLB (from left to right) results in a gradual reduction of gene expression (white) (Credit: Mann Lab/Columbia's Zuckerman Institute).
Only 25 of the 17,575 motif-based constitutive CTCF binding sites were farther than 70 bp from the nearest constitutive CTCF binding sites identified by T-KDE.
Although a small increase in Po is shown with added nucleotide for Ca-inhibited channels, it is also not clear whether this effect arises from the action of nucleotide at the binding site identified in the crystal, or from a non-specific action.
The binding site identified here has several unique characteristics.
As expected, the most affected residues are around the lactose-binding site identified previously from X-ray-crystallographic studies [ 24], and these include many of the same residues affected by GRG binding to gal-1.
The transcription factor binding site data set contained both the binding sites directly identified from the canonical sequence, and the binding sites "recovered" by substituting previously identified SNP into the sequence.
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