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Further predicted connections could be associated with potential regulatory binding sites generated from sequence data (Transfac, Ensembl).
Therefore, we used the consensus binding sites generated from FOXA1 ChIP-Seq data in A549 cells and hepatic HepG2 cells to narrow down a set of EMT-related and potentially FOXA1-regulated genes.
We found very few (<10) binding sites generated from some CLIP-seq data sets because of low sequencing quality and depth (usually less than 1 M unique mapped reads).
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A pharmacophore model of oxindole analog binding at the FGFR1 binding site generated from virtual screen results in a study by Kammasud then revealed that introduction of a phenyl hydrazide motif to C 5) of oxindoles proved to be the best possible to allow additional hydrogen bonding interactions with ATP site of receptor tyrosine kinases (RTKs), such as FGFR-1, VEGFR-2, PDGFRβ, and EGFR [20].
The AICD-binding sites generated from the two data sets were mapped on the genome relative to the nearest miRNAs and annotated with respect to their distance from the miRNA stem loop start sites (SSS; Supplementary Tables S1 and S2).
Comparisons of maps of such binding sites generated by the mouse and human ENCODE projects4,5,6,7 suggest that many differences in transcription levels between equivalent (orthologous) genes in the two organisms result from transcription-factor binding sites (labelled as TFs) occupying different locations.
Functional antibodies were obtained from a library of antigen-binding sites generated by a binary code restricted to tyrosine and serine.
A binding p-value was then determined for each genomic position by Wilcoxon rank sum test and binding sites were generated from those more significant than specified thresholds with a maximum gap of 500 and minimum run of 350.
The 'seed' deleted 3′UTR of CCL4 lacking of 7-nt of the binding site was generated from the WT reporter plasmid with primers (forward: 5′-tttctcgagCCAAAAGAAGCAAGC-3′ and backward: 5′-gagtggtgaccTAGACTTCCTGTCTCTGAGCAGC-3′).
Two different types of sets of example binding sites were generated for each cutoff score from 2 to 7. They correspond to sequences drawn from a step-function distribution or a Boltzmann distribution, in each case using a maximum energy cutoff for selected sites.
A genome-wide map of predicted STAT1 binding sites was generated as follows.
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