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Since aTf has two binding sites for iron, we explored the effects of ferric citrate and ferrous sulfate at 2.5 μM, a concentration that equalled the amount of binding sites on the added aTf.
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It is made up of two homologous halves each possessing binding site for iron [ 4– 8].
Another binding site for the iron-containing cofactor is most probably Cys176, which is located at the bottom of a deep intersubunit cleft and which has been shown to be essential for enzyme activity.
Genes required for iron homeostasis and pleiotropic drug resistance were also represented in this cluster, as were genes with promoter binding sites for Aft2p and Cin5p, which regulate resistance to oxidative stress (Table 1).
Furthermore two binding sites for bHLH factors were retrieved.
sharing binding sites for miRNAs.
PREs contain binding sites for sequence-specific DNA binding proteins.
McbR binding sites, for instance, indicate the connection of the tauABCD genes to the sulphur regulon of C. variabile, whereas DtxR binding sites within the siu and fiu gene clusters indicate the integration of the respective genes into an iron-dependent gene-regulatory network.
Potential binding sites for transcription factors inIL1R1and inIL1R1and
Together with the experimental results, we indicate a novel iron homeostasis regulating system sensing the amount of metabolically available iron and identify the binding site of iron-related SrbA target genes.
Iron is the binding site for ACC and oxygen.
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