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Scatchard analysis showed that two classes of binding sites existed in the SIN-imprinted polymers, with their dissociation constants estimated to be 7.257×10−5 and 3.828×10−3 mol l−1, respectively.
Thus, we performed sequence analysis to determine whether any putative E2F-1 binding sites existed in the PUMA gene promoter.
A comparison between the Sanger sequences and the reference 3'UTRs showed that nine SNPs were successfully detected by conventional sequencing [see Additional file 12: Figure S5. Fifteen SNP-type mRNAs were predicted to have 35 sites targeted by 27 miRNAs. Most of these binding sites existed in both the wild type mRNAs and SNP-type mRNAs.
Similar(56)
It has been shown that two putative ligand binding sites exist in SERT, named S1 and S2 [57 59], whereas a single substrate binding site is found in MAO-A [47].
Zheng et al. have hypothesized that several dozen potential miRNA binding sites exist in the genome-wide distribution of HPV (Harper and Demars, 2014), suggesting a need for further investigation to unveil more cellular miRNA-mediated regulation of HPV.
Our results show that high-quality potential miRNA binding sites exist in all regions of mRNAs.
Consistent with a role for miRNA, several putative miRNA binding sites exist in this region, however, none of these predicted miRNAs was regulated by inhibition of GSK3β (Figure S2).
It may also support the hypothesis that multiple target-protein binding sites exist in the αB-crystallin sequence, which are affected to a greater or lesser degree by conformational change.
On the other hand, Yhp1 shows the lowest in both entropy and linker ratio, suggesting that its binding sites exist in closed and stable chromatin.
The five Qrr sRNAs are controlled by five distinct promoters and hallmarks suggestive of different transcription factor binding sites exist in each promoter (Tu and Bassler, 2007).
In fact, the numbers of predicted binding sites in the three genomes are not too small (Table S27, S28, S31 in Additional file 5), which suggests that a few putative LexA-like binding sites exist in these genomes.
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