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However, many of these putative PhoP-binding sites are likely to be false positives because many sequences containing the putative binding sites did not bind PhoP when they were assayed by ITC (Table S1 of the Supporting Information).
The results show that more ZF-binding sites yield higher levels of GFP expression, whereas changing the spacing between binding sites did not significantly improve levels of expression.
The C/EBPβ binding site had transcriptional activities whereas the HIF1α binding sites did not have transcriptional activities under cAMP stimulation.
Compared with SPR measurements, the non-specific binding caused by the polymer matrix and/or randomly located fluorescent monomer residues that did not compose specific binding sites did not contribute to the observed fluorescence change.
We found that promoters with nine binding sites expressed GFP at higher levels than promoters with three binding sites when induced, whereas increasing the spacing between binding sites did not significantly improve levels of expression (Fig. 3). Figure 3: Optimization of ZF-binding sites (triangles) in the minimal promoter for estradiol-inducible expression.
Mutation of the gamma-globin gene promoter GATA-1 binding sites did not decrease either promoter strength or enhancement of activity by 5'HS2.
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Although CLIP approaches can identify non-canonical sites that bind the miRNA with some degree of specificity, these non-canonical binding sites do not function to mediate detectable repression.
In their case, only the methylated CpG sites were considered, however, typical GR binding sites do not have CpG site35.
According to Istvan et al.[17] the orientation of the side chains in the binding sites does not differ among the statins.
Deletion of these binding sites does not inhibit the function of Y RNAs during chromosomal DNA replication in vitro and in vivo [7], [10].
This leads us to conclude that the occupation of PDE non-catalytic binding sites do not explain the increase of τD in low Ca2+.
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