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We began model building by using experimentally-derived SigB binding sites derived from Bc ATCC14579 [ 44, 45].
Table 1 summarizes the ability of SiteHopper to detect structural similarity between analogous binding sites derived from structures of the same protein, for both the ligCav and fpocket-derived datasets.
We show that such plots can highlight the similarity between binding sites derived from different proteins.
Similarity between potential binding sites derived from different protein targets, cationic trypsin and prothrombin, is highlighted in red.
We present a workflow for the objective mapping of potential protein ligand binding sites derived from the currently available structural proteome.
Here, we present a method for generating a structural map of potential small molecule binding sites derived from the currently available structural proteome [3, 4].
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Binding site specificity was confirmed by EMSA in the presence of increasing amounts of non-specific (unrelated) double-stranded oligonucleotide or AmphiFoxD binding site derived from AmphiVent2 promoter region.
To determine if the CREB element can regulate PP2A-Aα promoter, we also conducted gel mobility shifting assay with an oligo primer containing the CREB binding site derived from the PP2A-Aα promoter (Fig. 4A).
The Grb2-specific Tpr-Met variant (TM-Grb2) contains the Grb2 binding site derived from EGFR.
Binding reactions with Nv Msx proteins were performed on a consensus Msx/Pbx binding site derived from vertebrates and containing an additional Meis binding site in a topology similar to the Hox probe ('Materials and methods').
We next investigated, by comparing wild-type and E1A/Ras cells, which the NF- κB member may interact with the κB binding site derived from A20 promoter using oligonucleotide pull-down assay.
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