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Combinative use of more than one computational algorithm in the prediction of miRNA binding sites could decrease the false positive rate, but it might increase the false negtive rate because the overlap between computational algorithms is not very good.
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Dual-luciferase assay clearly demonstrated that decreased or deleted NF κB binding sites could downregulate or even block the doxorubicin-induced Notch1 transcription; these results suggest that NF κB has a positive regulation role on Notch1 transcription.
Such interactions between elements around the binding sites could easily modulate affinity resulting in autoinhibition.
Identification of binding sites could facilitate design of DNA binding ligands for capture and detection of insulin and IGF-2.
The cross-linked binding sites could be adsorbed to wheat germ agglutinin and to anion exchange resins.
For this kind of bound structures, binding sites could not be computed directly based on their ligands.
Neither cytotoxicity nor the competition between TCC and testosterone for binding sites could be observed in their studies.
The data suggest that single nucleotide polymorphisms (SNPs) in individuals or populations that may introduce substitutions in CEACAM sequence particularly at the bacterial binding site, could not only decrease but also significantly increase the functional affinity of pathogen interactions.
For example, a transcriptional suppressor protein that competes with a transcriptional activator for the same binding site could result in decreased activation through increased methylation [13].
Either visfatin or molecules that fit visfatin's binding site could help control the disease.
This fortuitous binding site could later prove to be a useful binding site for other low molecular mass partners.
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