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Finally, we were able to identify common EN/GsbN binding sites corresponding to loci in which EN-regulated genes have been previously identified [18], [19], [21].
Structural and functional analyses of 18 mutants revealed two independent binding sites corresponding to the dual activities of HWTX-XI: strong inhibition of serine proteases and weak inhibition of Kv channels.
To demonstrate a direct interaction between miR-155 and the FOXO3a 3'-UTR, we sequentially mutated these four binding sites corresponding to the miR-155 seed regions (Figure 4B).
In other words, as N increases, the number of genes with products corresponding to a binding site of gene i increases (in-degree), and the number of genes with binding sites corresponding to the product of gene i increases (out-degree).
This fact makes it difficult to detect all the binding sites corresponding to a global regulator without a specific definition of their binding sequence on the DNA.
An interesting aspect of this bioinformatic analysis is the detection of several potential AtoC binding sites, corresponding to the pattern of motif 1, within gene-encoding regions.
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For comparison, the previously determined apo-Gal3C structure included only three water molecules in the binding site (corresponding to O3, O4′, and O6′).
These and related findings were explained by proposing two competing binding sites for long-chain QAs: a hydrophilic headgroup binding site (corresponding to the internal TEA site) and a hydrophobic tail binding site.
We also compared overlaps between 5hmC differences and publicly available (Encode) H3K4me1- and H3K27ac-binding sites that are enriched at enhancers.51 We detected 1788 probes at H3K4me1-binding sites corresponding to 912 genes, and 631 probes at H3K27ac-binding sites corresponding to 357 genes (Supplementary Tables S7-8).
Using the 20 base pair Pax6 PD "consensus" sequence [36] and a 20 bp Pax6-specific derivative of the 17 base pair PHO (PD/HD) Drosophila paired "consensus" [37] described in Methods and in Fig. S10, we identified five putative Pax6-binding sites corresponding to the "peak" regions identified by ChIP-on-chip in Mab21l2 and seven Pax6-binding sites in Tgfb2 loci, respectively.
Our ChIP-Seq datasets resulted in the identification of 286 high confidence REV-binding sites (corresponding to 552 potentially regulated genes) across the entire Arabidopsis genome (Brandt et al., 2012).
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