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(B) Structure of HEF binding sites complexed with the receptor.
(A) Superimposing of HEF and HA receptor binding sites complexed with ligand (9-acetamidosialicacid α-methyl glycoside).
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Ligplot (Laskowski and Swindells, 2011) drawings of both the known and inferred binding sites in complex with the drug are available as well (Fig. 1).
Myc is a nuclear transcription factor that exhibits high-affinity and site-specific DNA-binding activity when complexed with its cellular partner Max, and it is rate-limiting for cell cycle progression through G1 phase [ 11], mediated in part through its effects on activation of cyclin-dependent kinases [ 12, 13].
To visualize the low-affinity nickel binding sites when NikR is complexed with DNA, we soaked stoichiometric Ni2+−NikR−DNA crystals with excess nickel chloride and collected data at the nickel peak wavelength (1.4845 Å).
The lower affinity of F1P for GK-bound GKRP is likely the result of an altered phosphate ester binding site on GKRP when complexed with GK.
A promoter region is typically a few thousand base pairs long and contains many different binding sites with often complex interactions.
Brk accomplishes this via repressor complexes containing Teashirt that compete for enhancer binding sites with Arm/dTCF activation complexes [29].
The experimental results suggests that the strength of the complex binding sites decreases considerably with increasing [Ca2+] and that a single H2O2 molecule is required to combine with one available active binding site.
It was further noted that multiple Hap complex binding sites are associated with induction (Fig. 3A).
Expansions via transposable elements are increasingly recognized as a general mechanism for the generation of new binding sites of TFs with complex binding motifs (Johnson et al., 2006, 2009; Mortazavi et al., 2006).
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