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No significant difference was detected in the percentage of binding sites captured from tissue samples of WT and HET α4 (79.6 ± 5.4 vs 83.1 ± 7.5%; n = 3, P = 0.72, t-test) or β2 (79.3 ± 0.9 vs 78.8 ± 1.3%; n = 3, P = 0.78, t-test), indicating that immobilized mAb295 performed similarly in all samples regardless of nAChR protein content.
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There was no significant binding site capture from brains of α4 KO or β2 KO mice.
In mammalian cells, most cohesin-binding sites captured by chromatin immunoprecipitation (ChIP) were first reported to be DNaseI hypersensitive sites also bound by CTCF (25– 25).
CLIP analysis has been successfully used to study the murine RNA binding protein NOVA, and also reveal the specific binding sites within the captured pre-mRNAs [ 7, 8].
In this case, the excessive protons could capture the binding sites of active groups from Pt species; which would cause rather part of Pt(0) to be separated from the biomass and no longer sheltered by the biological macromolecules.
g, Motifs identified within BCL11A binding sites from BCL11A ChIP seq in HUDEP-2 cells.
(B) A conserved SigB binding sites were found from 29 TSSs (p-value of 1.51 × 10−8).
Watanabe, Y. H., Motohashi, K. & Yoshida, M. Roles of the two ATP binding sites of ClpB from Thermus thermophilus.
This motif contains the core element of binding sites from the Ets family of transcription factors.
*genes preceded by predicted FurB binding sites from FIMO analysis.
We find that human and chimp FOXP2 have similar binding sites that are distinct from previously suggested consensus binding sites.
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