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Binding constant and the number of binding sites can be calculated by Stern Volmer equation, modified Stern Volmer equation, Lineweaver Burk equation, Benesi Hidebrand equation, Benesi Hidebrand equation and multiple binding sites equation.
The interaction of most ligands with their binding sites can be characterized in terms of binding free energy or binding energy (BE).
Sometimes, information about binding sites can be found in SELEX [ 36, 37] or Protein Binding Microarray (PBM) experiments [ 38].
Substantial similarity of EA receptors regarding their binding sites can be assumed.
These binding sites can be considered as prerequisite or allosteric binding sites.
These binding sites can be oriented to model the monomeric architecture of polymers.
In addition, the electrostatic potential binding energy and similarity between the binding sites can be calculated based on the binding pose of the ligand.
Some fluorescent probes have specific binding to different regions of the HSA, and the binding sites can be determined by the displacement binding experiments using some probes.
Cis-regulatory elements such as transcription factor (TF) binding sites can be identified genome-wide, but it remains far more challenging to pinpoint genetic variants affecting TF binding.
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Upon engineering the C-terminal loops in the CH3 domains, Fcabs (Fc domain with antigen-binding sites) can be designed.
Further analyses suggested that zinc-binding proteins are preferentially implicated in a variety of diseases and highly enriched in known drug targets, and the prediction of zinc-binding sites can be helpful for the investigation of molecular mechanisms.
More suggestions(18)
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