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Even in cases where the properties have been studied extensively, the binding sites attributed to an important protein such as SF2/ASF depend on whether they are assayed with pure protein (Tacke and Manley, 1995), functional selection (Liu et al, 1998; Smith et al, 2006) or cross-linking in cells (Sanford et al, 2008) and in all cases they are diverse.
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On the possible route towards DRTs described in Appendix 1, the appearance of concatenation of multiple amino acid binding sites was not attributed to the reduction of the replication cost, but to the efficiency of the replication.
Long-duration binding might be attributed to strong binding whereas frequent binding/unbinding would not be.
Notably, protecting amine groups did not affect biotin avidin binding, which may be attributed to a unique conformation of biotin binding site composed of a conserved Trp120, a hydrophobic pocket, and eight hydrogen bonds, while lacking exposed amines.
Its unique biphasic Zn II -binding behavior could be attributed to the presence of two distinct Zn II -binding sites and allowed ratiometric fluorescent detection of Zn(II) over a concentration range from 10 nM to 1 mM.
Oxidoreductases have more non-polar active sites, which can be attributed to cofactor binding and a preference for Glu over Asp in active sites in comparison to the other classes.
For fully covered small nanoparticles the IR bands are attributed to all binding sites.
While we know that the flanking GAGA sites are important for early boundary activity, we haven't detected EMSA shifts that could be attributed to the GAGA binding sites either in early or late nuclear extracts.
We further performed detailed analysis on the amino acid contributions to the binding energies and found that the strong binding affinity for 6F-tricin to CDK9 is attributed to the specific binding orientation of 6F-tricin in the ATP-binding site.
As seen in Fig. 5, biosorption has been observed to increase as initial concentration increases; this may be attributed to the active binding sites available for available sorbate ions [24].
This correlation was attributed to fewer miRNA binding sites in the shorter 3'UTR isoforms of an mRNA leading to higher mRNA stability and expression.
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