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Combinatorial regulation by multiple TFs is less well-known and fewer combinatorial annotated binding sites are reported in databases or even in the literature (see [79]).
Although p53 is known to bind to sites with a spacer as high as 13 bp [ 75], for the current analysis we considered 4 bp spacer as the maximum limit because majority of the validated p53 binding sites are reported to have a spacer less than 4 bp (see Additional Files 1 and 2 for the putative p53-miR distribution).
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Four binding sites were reported in the crystal structure of mouse ABCB1 as represented by the following sites: ABCB1-QZ59-RRR (site-1), ABCB1-QZ59-SSS (site-2), ABCB1-verapamil (site-3) and the site common to the above three sites (site-4) [27].
References to the publications where protein-protein interactions and consensus binding sites were reported are included.
Native β-lg has one main well-defined binding site per monomer, and depending on the ligand and medium conditions, one or more secondary binding sites were reported.
Enriched functions, such as common gene ontology, biological pathways, shared transcription factor or miRNA binding sites, were reported using the default g:SCS method for significance threshold determination.
These binding sites were reported to relate to transcription activation in B. subtilis ([ 15, 19, 38, 52, 57- 59] and raw data file 1) and are supposed to be TnrA-specific as GlnR has not been reported to activate transcription.
Based on the above-mentioned two datasets, Bayesian Neural Networks was used to perform the classification and the MPCC on the test data sets (the half-available binding sites) was reported, Table 1 and Additional file 1 Table S1a.
There are 10 ATP-binding sites and one active site residue of which 8 ATP-binding sites are reported amongst somatic mutations responsible for lung cancer.
A monovalent streptavidin tetramer with only one functional biotin binding site was reported [16]: The unmodified and modified monomers were combined in a denaturated state at a molar ratio of 1∶3 before refolding.
Large sets of genes are also repressed under environmental stress in Sc and Sp respectively [ 31, 32] and the same TF binding site was reported in both systems.
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