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Non-promoter CTCF binding sites are often located in a linker region between precisely positioned nucleosomes52 that makes them more vulnerable to the DNA topology changes induced by curaxins.
Additionally, important sequence features, such as interactions among adjacent binding sites, are often not represented in these approaches.
As miRNA binding sites are often located close to polyadenylation signals (PAS) [60], shortening of the 3'UTRs may lead to loss of miRNA binding sequences and thus loss of microRNA-mediated regulation.
The observation that Sp1 binding sites are often present in CGIs is not new [ 10].
TF binding sites are often clustered together, called cis-regulatory modules (CRMs).
The actual binding sites are often short sequences embedded in these peak sequences.
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Drug discovery for difficult targets that have large and flat binding sites is often better suited to compounds beyond the "rule of 5" (bRo5).
Experimental determination of atomic resolution structures of ligands weakly bound to multiple binding sites is often challenging.
The '-35 box' of sigma 70 binding sites was often significantly degenerate, and therefore difficult to detect [ 14].
From the analysis of several protein protein complex structures, the most obvious observation was that protein surfaces of interacting proteins at binding sites were often highly complementary.
Using ChAP-seq, binding sites were often detected as two broad peaks on the forward and reverse strands, as previously reported.
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