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These two binding sites are involved in adipocyte differentiation [ 24].
We here show that multiple transcription factors and binding sites are involved in transcriptional regulation of the LM-332 genes.
Thus, it appears that only a small subset of CREB binding sites are involved in conveying the cAMP signal to control gene expression in the liver, despite the observation that the majority of CREB sites are in known promoter regions.
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The mechanism of the A/D transition of the enzyme is far from being understood, however one of the accessory subunit, 39 kDa (NDUFA9) and two mitochondrially encoded subunits, ND3 and ND1, all located in the area of the quinone binding site are involved in conformational rearrangements.
The general analysis presented here should be applicable to most surface reactions that involve ligand-receptor binding wherein multiple-binding sites are involved on either the receptor or the ligand.
To investigate whether this putative EGR3 binding site and the identified SRF and TFAP2-binding sites are involved in transcription of frataxin, we generated four luciferase reporter constructs.
It is known that cis-elements other than DOF-binding sites are involved in the regulation of guard cell expression.
Interestingly, the only exception in our setting of experiments was MCP-1, wasch was released after S100A8/S100A9 stimulation irrespective of whether cells were preincubated with AGEs or not, implying that receptors different from RAGE and/or other AGE-binding sites are involved in S100A8/S100A9 dependent MCP-1 expression.
To determine whether the putative c-Jun binding site is involved in TNC transcription, we constructed reporter vectors with the DNA sequence of the TNC promoter containing a wild-type (TNC-wt) or mutated (TNC-mut) putative c-Jun binding site (Fig. 5I).
This pattern indirectly implicates the P-domain and suggests that a conformation-dependent binding site is involved.
When more than one binding site is involved in gene regulation, as in higher eukaryotes, their collective formal description is more elaborate.
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