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When different types of binding sites are introduced, one should be careful to check whether especially the diffusion rates obey this detailed balance criterion.
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Thus, pMEF2FLuc, a construct containing luciferase downstream to a promoter with MEF2 binding sites, was introduced into HeLa cells.
Mutations of potential trans-regulator binding sites were introduced in one of the truncated msn DNAs using the QuickChange site-directed mutagenesis kit from Stratagene.
A 4× version of the miR-312 binding sites was introduced into the XbaI site between the osk coding region and UASp vector 3' UTR of UAS-osk [62] to make UAS-osk-312 (p8619).
Decoy oligodeoxynucleotides bearing consensus transcription factor binding sites were introduced into chondrocytes to determine the functionality of our results.
Inhomogeneity in terms of binding sites was introduced to increase the temperature range in which H2 can be formed toward higher temperatures.
De novo Zn II -binding sites were introduced directly at the surface of both fluorescent domains of a chimera of enhanced cyan and yellow fluorescent protein, connected by a flexible peptide linker.
When a potential miR-222-binding site was introduced, as is the case in the ETV1long construct, miR-222 overexpression led to a small but significant decrease (12%, P=0.02) in the luciferase activity.
PmeI and NheI restriction sites were introduced in the primer as cloning sites for NR ligand binding domains.
For GST-free RAP used in binding assays, a TEV cleavage site was introduced between the GST and RAP moieties.
An XcmI site was introduced downstream of the AflII site.
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