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Then, this binding site is checked for phylogenetical conservation.
Additionally, citrullination sites were checked manually.
(GeneCodes, Ann Arbor, Michigan) and variable sites were checked visually for accuracy.
Mutation of the binding consensus site was checked by sequencing, and the fragment was purified by the silica-based Gene Clean II kit (MP Biomedicals, Cleveland, OH) and ammonium acetate DNA precipitation method.
Orientation of the LoxP site was checked by sequencing.
When different types of binding sites are introduced, one should be careful to check whether especially the diffusion rates obey this detailed balance criterion.
Here, these binding sites are not discussed one by one.
These binding sites are highly charged.
Noteworthy, these binding sites are juxtaposed to GATA3-binding sites.
All the designed mutant nucleotide sequences were re-analysed in Match 1.0 Public to check for loss of the specific transcription factor binding site and that no new binding sites were generated.
Potential binding sites were elucidated by docking.
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