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The correlations between TF abundance and DNA methylation at their binding sites are calculated with Spearman rank correlation and therefore the P-values are expected to behave appropriately.
Residues undergoing changes in binding sites are calculated using a rotamer-based approach as well as the Δ χ threshold of 60° used in our previous study (Najmanovich et al., 2000).
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The cavity volume at the binding sites was calculated, using DS (Binding Site module) for the coordinates obtained from protein data bank (c-Src, PDB ID: 1YOL, 1Y57, 1FMK, 2H8H and for c-Abl, PDB ID: 1M52 and 2FO0).
Binding free energies of probes that bind strongly to the RNA at the predicted binding sites and potential alternative binding sites were calculated at 4, 22, and 37 °C using the nearest neighbor model and 2′- O-methyl RNA/RNA parameters with bonuses for LNA modifications (37− 40).
The quenching rate constants, binding constants, and number of binding sites were calculated in the presence of CSB.
Dissociation constants and concentration of binding sites were calculated using the LIGAND program [56].
Then, the nucleosome occupancy surrounding the binding sites was calculated.
The number of ADP binding sites was calculated.
For the 25TAC inhibitor, the number of binding sites was calculated to be 36 [2 × (25 – 8 + 1) = 36].
The intensity map for Dendra2 Pol II or stable Sox2 binding sites was calculated by 2D Gaussian kernel density function implemented by Matlab.
The total number of cortactin filament side binding sites was calculated by assuming a cortactin to F-actin subunit stoichiometry of 1 6.
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