Sentence examples for binding sites are blocked from inspiring English sources

VDAC1 will lose its Ca2+ transport capacity when the Ca2+ binding sites are blocked by Ruthenium Red (RuR) (Gincel et al., 2001).

The immunoplate in this kit is precoated with secondary antibody, and the nonspecific binding sites are blocked.

Usually the ligand is coated onto a microwell at high pH after which any remaining binding sites are blocked with PBS containing 0.05%Tween200.05%Tween20

The extent of condensation was smaller as compared to that in the NC probes, and this distinction may arise because a large proportion of the nucleobase-cluster binding sites are blocked by hybridization.

In the motif-free conformation first shown for AP2 and subsequently for AP1 (Heldwein et al., 2004), both cargo-motif binding sites are blocked by portions of the β2 subunit: β2Tyr6 and β2Phe7 block the [ED]xxxL[LI]-binding site and β2Glu364 β2Val406 (especially β2Val365 and β2Tyr405) block the YxxΦ site (numbering refers to AP2), and thus we term this the "locked" conformation.

After further washing, the nonspecific binding sites are blocked by incubation in a blocking solution of BSA (1 % w/v), 0.2 % Triton X-100 (v/v) in PBS supplemented with Tween-20 (0.05 % w/v) (PBST) for 45 min at room temperature.

The residual binding sites were blocked through incubation in TBS containing 0.2% Tween and 5% BSA.

Unspecific binding sites were blocked by incubating membranes in phosphate-buffered saline containing 0.5%Tween-20Tween-20and5% or bovine serum albumin.

After washing, unoccupied binding sites were blocked with blocking buffer (0.1 M Tris-HCl, pH 8.0) at room temperature for 2 h.

Nonspecific binding sites were blocked by incubating with 5% non-fat milk, and then the membranes were incubated with primary antibodies.

Then, excess binding sites were blocked with 0.5% (w/v) BSA in 10 mM PBS (pH = 7.4) for incubating overnight at 4 °C.