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Synthetic oligonucleotides were ligated to create altered CtrA binding sites and corresponding promoters with varied transcription strength.
They hope to identify the complete set of DNA binding sites and corresponding target genes for the regulons in embryonic stem cells and a subset of the cells they differentiate into.
The obtained quenching mechanisms, binding constants, binding sites and corresponding thermodynamic parameters at different temperatures indicate that the hydrophobic interaction play a major role in the morin-BSA association.
The more ubiquitous transcription there is, the more likely the predicted binding sites and corresponding modules are functional, and the closer the findings we report below are a true reflection of reality.
If the regulatory regions of specific genes are studied, as will be the case here, high utilization of binding sites means that many predicted binding sites and corresponding modules are functional, even though many of them are only functional in specific, often negligible contexts.
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The binding constants, binding sites and the corresponding thermodynamic parameters (ΔH, ΔG and ΔS) were calculated.
We first identified the most stable binding sites and their corresponding binding energies for a single Na or Li adatom on the considered membranes.
In both cases, the PLS model Score was superior in discriminating between true known liganded binding sites, and those corresponding to cavities without ligands bound.
The binding constants, binding sites and the corresponding thermodynamic parameters (ΔH, ΔG and ΔS) were determined, which indicates that hydrogen bonding and van der Waals forces play a major role in the binding process.
This strategy has yielded strong evidence of a direct link between the gain/loss of CTCF binding sites and a corresponding gain/loss of local domain insulation.
Schematic representations of 3′UTRs of cytoskeletal genes (A ) Cfl2, (B ) Wasl, (C ) Bod1, Twf1, (E ) Itgav, (F ) Grfl1 with miR-142-3p miR-142-3p miR-142-3p corresponding mutations made to test direct interactions with miR-142-3p.
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binding sites and microRNA
binding sites and binding
binding sites and specific
binding genes and corresponding
binding sites and key
binding sites and few
binding sites and single
binding profiles and corresponding
binding sites and strong
binding constants and corresponding
binding sites and positive
binding sites and other
binding sites and functional
binding sites and regulatory
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